Anti-oxidative effect of mangiferin-chitosan nanoparticles on oxidative stress-induced renal cells

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Oxidative Stress Research

Background:

• Oxidative stress is a major contributor to chronic diseases.
• Antioxidant therapy shows promise but faces challenges with bioavailability.
• Mangiferin (MGF), a polyphenol, has antioxidant properties but poor solubility limits its in vivo efficacy.

Purpose of the Study:

• To develop mangiferin nanoparticles (NPs) to enhance MGF solubility and bioavailability.
• To evaluate the therapeutic efficacy of MGF NPs against oxidative stress.
• To investigate the protective effects of MGF NPs on normal kidney epithelial (NKE) cells in a nephropathic model.

Main Methods:

• Formulation of mangiferin-chitosan nanoparticles (MCNs).
• Assessment of free radical scavenging activity in cell-free systems.
• Evaluation of synergistic effects with antioxidant enzymes (catalases, peroxidases).
• Testing MCNs in a sodium fluoride (NaF)-induced nephropathic model using NKE cells.

Main Results:

• MCNs demonstrated significant free radical scavenging activity.
• Nanoparticles showed synergistic action with phase II antioxidant enzymes.
• Pre-treatment with MCNs prevented NaF-induced cytotoxicity in NKE cells.
• MCNs maintained intracellular antioxidant enzyme levels and inhibited lipid peroxidation and protein oxidation.

Conclusions:

• Mangiferin-chitosan nanoparticles effectively improve mangiferin's bioavailability and solubility.
• MCNs exhibit potent antioxidant and cytoprotective properties against induced oxidative stress.
• MCNs hold potential as therapeutic agents in the food and pharmaceutical industries for preventing oxidative stress-related disorders.