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A peanut agglutinin binding glycoprotein in CNS myelin and oligodendrocytes
D D Mikol1, S Szuchet, K Stefansson
1Department of Neurology, University of Chicago, Illinois 60637.
Annals of the New York Academy of Sciences
|January 1, 1988
Abstract:
We isolated and characterized a 120-kd PNA-binding polypeptide from the human CNS. This polypeptide is linked to membranes through a PI linkage. After release from membranes by PLC it measures 105 kd, 30 kd of which appear to be contributed by N-linked carbohydrates. We isolated the polypeptide by the use of PLC and PNA affinity chromatography and used it to raise polyclonal antibodies and to determine the N-terminal sequence. Immunohistochemical and immunochemical studies using these antibodies showed that 120 kdpp is present in both myelin and oligodendrocytes.