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Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization
Xiaohua Zhang1,2,3, Senjie Lin4, Dongyan Liu5
1Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Environmental Microbiology
|February 21, 2020
Summary
Marine diatoms like Skeletonema costatum can efficiently use adenosine-5'-triphosphate (ATP) as a phosphorus source, similar to dissolved inorganic phosphorus (DIP). This study reveals novel molecular mechanisms for ATP utilization in diatoms.
Area of Science:
- * Marine microbial ecology
- * Biogeochemical cycles
- * Phytoplankton physiology
Background:
- * Dissolved organic phosphorus (DOP) is a crucial nutrient source for phytoplankton when dissolved inorganic phosphorus (DIP) is scarce.
- * Phytoplankton's ability to utilize DOP influences their competitive advantage in aquatic ecosystems.
- * Adenosine-5 '-triphosphate (ATP) is a significant component of DOP in marine environments.
Purpose of the Study:
- * To investigate the physiological and molecular mechanisms by which the marine diatom Skeletonema costatum utilizes ATP.
- * To compare the efficiency of ATP utilization with DIP for S. costatum growth.
- * To identify genes and metabolic pathways involved in ATP assimilation.
Main Methods:
- * Incubation experiments with S. costatum under conditions of ATP, DIP-replete, and DIP-depleted media.
- * Growth rate measurements to assess the utilization efficiency of ATP and DIP.
- * Transcriptome analysis to identify differentially expressed genes under ATP utilization.
Main Results:
- * ATP supports the growth of S. costatum with comparable efficiency to DIP.
- * ATP utilization by S. costatum does not involve alkaline phosphatase (AP) but does involve extracellular hydrolysis.
- * Upregulation of hydrolase-related transcripts (e.g., NAD+ diphosphatase) and downregulation of purine metabolism genes were observed in ATP-grown cells.
Conclusions:
- * Skeletonema costatum can effectively utilize ATP as a phosphorus source, highlighting its ecological significance.
- * The diatom employs unique molecular strategies, distinct from AP hydrolysis, for ATP assimilation.
- * Transcriptomic data reveals adaptive metabolic adjustments in S. costatum when utilizing ATP.
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