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Updated: Feb 11, 2026

Determining Binding Affinity KD of Radiolabeled Antibodies to Immobilized Antigens
Published on: June 23, 2022
[Determination of the concentration of antibody reactive centers using a standard antigen and erythrocytic antigen]
Abstract:
The concentration of the reactive centres of the antibodies in the preparation under study can be determined with the aid of an antibody erythrocytic diagnostic agent. A mathematical substantiation of the suggested method and definite examples of its use are presented. The sensitivity of the serological method of determination of the concentration of the reactive centres of the antibodies largely depended on the valency of the antibody erythrocytes and the heterogeneity of the antibodies. This method permits to determine the sum of the reactive antibody centres equivalent to the definite concentration of the molecules of the standard antigen whose valency remains unknown.
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