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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Related Experiment Video

Updated: Dec 28, 2025

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

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An aptamer-based, fluorescent and radionuclide dual-modality probe.

Gui-Xiong Zhang1, Yan-Lan Liu2, Min Yang1

  • 1Department of Nuclear Medicine, The Third Affiliated Hospital of Sun Yat-sen University, 600 Tianhe Road, Guangzhou, 510630, China.

Biochimie
|February 22, 2020
PubMed
Summary

Researchers developed a novel dual-modality aptamer probe for cancer imaging. This probe combines fluorescent and radionuclide properties, showing specific binding to hepatoma cells for potential theranostics.

Keywords:
AptamerCarboxyfluorescein (FAM)Dual-modalityIodine-131

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Area of Science:

  • Biotechnology
  • Molecular Imaging
  • Nucleic Acid Chemistry

Background:

  • Aptamers are effective molecular probes for targeted delivery of imaging or therapeutic agents.
  • Developing dual-modality probes enhances diagnostic capabilities and theranostic potential.

Purpose of the Study:

  • To create a novel dual-modality (fluorescent and radionuclide) probe using a truncated aptamer.
  • To evaluate the in vitro stability and binding affinity of the developed probe.

Main Methods:

  • Truncation of aptamer JHIT2 to create JHIT2e using RNAstructure and mfold software.
  • Conjugation of carboxyfluorescein (FAM) and Iodine-131 to the truncated aptamer JHIT2e.
  • In vitro cell uptake and fluorescence imaging assays using HepG2 cells.

Main Results:

  • The truncated aptamer JHIT2e retained specificity and affinity for HepG2 cells.
  • The dual-modality probe 131I-FAM-JHIT2e exhibited both fluorescence and radioactivity signals.
  • The probe demonstrated specific binding to the human hepatoma cell line HepG2 in vitro.

Conclusions:

  • A novel aptamer-based dual-modality probe (131I-FAM-JHIT2e) was successfully developed.
  • The probe maintains specific binding to HepG2 cells, suitable for dual-modality imaging.
  • This work provides a foundation for aptamer-based theranostic agents.