Related Experiment Video
Updated: Dec 28, 2025
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Evaluation of cell and hemocompatibility of Cucurbiturils
Sarah Fink1, Kirsten Reddersen1, Cornelia Wiegand1
1Department of Dermatology, Jena University Hospital, Jena, Germany.
Background:
Cucurbiturils (CB) are pumpkin-shaped macrocyclic molecules consisting of methylen-bridged glycoluril units. Because of their complexing characteristics, they can be used as drug containers for medical purposes. For future biomedical and dermal application of CB, the investigation of cell compatibility is essential. Little is known about the influence of CB on eukaryotic cells, especially on dermal keratinocytes. The structurally related cyclodextrins are known to induce cell death by apoptosis in HaCaT keratinocytes as well as hemolysis in erythrocytes.
Objective:
To examine cytotoxic effects of different CB.
Methods:
Different cytotoxicity tests were performed on HaCaT keratinocytes and erythrocytes incubated with CB[5], CB[6], and CB[7].
Results:
CB[5] and CB[6] did not lead to cytotoxic reactions at high concentrations up to 30 mg/mL whereas incubation with CB[7] triggered apoptosis at a concentration of 3.75 mg/mL. None of the investigated CB caused hemolytic effects on erythrocytes.
Conclusion:
These results confirm the high potential of CB as host-complexes for biomedical and dermal applications.
