Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enlargement of the Plasma Membrane

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Direct visualization of native GSDMD pores reveals lipid-driven stabilization during pyroptosis.

Science advances·2026

Same author

Effects of Ultrasound-Mediated Treatments on Dental Biofilm Attachment and Viability.

Ultrasound in medicine & biology·2026

Same author

3D bone printing via ultrasound-mediated osteogenic differentiation of stem cells (3DBonUS).

Biofabrication·2026

Same author

Perfluorocarbon nanodroplets are cytocompatible with osteoblast-lineage cells and modulate in vitro osteoclastogenesis differentially in normoxia and hypoxia.

Scientific reports·2026

Same author

Smart event-triggered MINFLUX microscopy to catch and follow rare events.

Nature communications·2026

Same author

Microbubbles manufactured using a tissue homogeniser: DoE-guided optimisation for a narrower size distribution and higher yield.

International journal of pharmaceutics·2026

Search research articles

Related Experiment Video

Updated: Dec 28, 2025

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure.

Erdinc Sezgin^1,2, Dario Carugo³, Ilya Levental⁴

¹MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK.

|February 23, 2020

Summary

Researchers developed supported plasma membrane bilayers (SPMBs) from giant plasma membrane vesicles (GPMVs) using microfluidics and ultrasound. This method preserves molecular mobility, offering a new model for studying cell membrane dynamics.

Keywords:

GPMVs acoustic pressure plasma membrane bilayers plasma membrane vesicles supported bilayers

More Related Videos

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Published on: December 1, 2015

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

Related Experiment Videos

Last Updated: Dec 28, 2025

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Published on: December 1, 2015

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

Published on: January 16, 2019

Area of Science:

Biophysics
Cell Biology
Materials Science

Background:

Model membrane systems simplify studying biological processes and physicochemical principles.
Giant plasma membrane vesicles (GPMVs) are intermediate models between live cells and artificial structures.
Planar membrane surfaces are required for specific experimental applications.

Purpose of the Study:

To develop a novel method for creating supported plasma membrane bilayers (SPMBs).
To utilize cell-derived GPMVs for generating planar membrane models.
To assess the accessibility and mobility of molecules within the generated SPMBs.

Main Methods:

Utilizing a microfluidic device for controlled GPMV manipulation.
Employing ultrasound to burst GPMVs and form supported bilayers.
Characterizing the SPMBs to evaluate molecular mobility and accessibility.

Main Results:

Successfully created supported plasma membrane bilayers (SPMBs) from GPMVs.
Demonstrated that the mobility of outer leaflet molecules is preserved in SPMBs.
Indicated that SPMB surface molecules are accessible for further studies.

Conclusions:

The developed method provides a novel approach to generate SPMBs.
SPMBs serve as valuable model systems for investigating plasma membrane dynamics.
These SPMBs offer potential for detailed characterization in various biophysical applications.