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Updated: Dec 28, 2025

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
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A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

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Circular RNA hsa_circ_0076906 competes with OGN for miR-1305 biding site to alleviate the progression of osteoporosis

Jian Wen1, Zhiping Guan1, Binsheng Yu1

  • 1Department of Spinal Surgery, Peking University Shenzhen Hospital, Lianhua Road, Shenzhen, 518000 Guangdong, China.

The International Journal of Biochemistry & Cell Biology
|February 23, 2020
PubMed

Insights

Circular RNA circ_0076906 promotes bone formation by regulating the miR-1305/Osteoglycin pathway, offering a potential therapeutic strategy for osteoporosis by enhancing osteogenic differentiation of human mesenchymal stem cells.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Regenerative Medicine

Background:

  • Osteoporosis is characterized by reduced bone formation and increased bone resorption.
  • Human mesenchymal stem cells (hMSCs) are crucial for bone regeneration due to their differentiation potential.
  • Circular RNAs (circRNAs) are emerging regulators in various biological processes, including bone metabolism.

Purpose of the Study:

  • To investigate the role of circ_0076906 in the osteogenic differentiation of hMSCs.
  • To elucidate the molecular mechanism involving circ_0076906, miR-1305, and Osteoglycin (OGN) in osteoporosis.
  • To evaluate the therapeutic potential of circ_0076906 in alleviating osteoporosis.

Main Methods:

  • Osteogenic differentiation induction in hMSCs.
  • Quantitative real-time PCR (qRT-PCR) and Western blot for gene and protein expression analysis.
  • Alkaline phosphatase activity assays, Alizarin Red staining, and luciferase reporter assays to assess bone formation and molecular interactions.

Main Results:

  • Circ_0076906 expression was upregulated during osteogenic differentiation.
  • Silencing circ_0076906 inhibited osteogenesis-related gene expression in hMSCs.
  • Circ_0076906 functioned as a molecular sponge for miR-1305, and miR-1305 regulated OGN expression.
  • The pro-osteogenic effect of circ_0076906 was dependent on the miR-1305/OGN pathway.

Conclusions:

  • Circ_0076906 promotes osteogenic differentiation of hMSCs.
  • The circ_0076906/miR-1305/OGN axis plays a critical role in regulating bone formation.
  • Circ_0076906 represents a potential therapeutic target for treating osteoporosis.

Keywords:
Circular RNAOsteogenic differentiationOsteoporosishMSCsmiR-1305/OGN pathway

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