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Updated: Dec 27, 2025
Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics
Published on: June 17, 2012
Chromatin Analysis of Metabolic Gene Clusters in Plants
Ancheng C Huang1, Hans-Wilhelm Nützmann2
1Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology, Shenzhen, China. huangac@sustech.edu.cn.
Abstract:
Plant metabolic gene clusters consist of neighboring genes that are involved in the biosynthesis of secondary or specialized metabolites. The genes within clusters are typically co-regulated, share a common set of chromatin marks, and code for the biosynthesis enzymes of a single metabolic pathway. Here, we describe three essential protocols for the basic analysis of metabolic gene clusters at transcription, histone modification, and metabolite level. The protocols are specified to clusters in the Arabidopsis thaliana genome and are transferable to other plant species.
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