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Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
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pH-Sensitive Mechanical Properties of Elastin-Based Hydrogels.

Sydney Hollingshead1, Julie C Liu1,2

  • 1Davidson School of Chemical Engineering, Purdue University, West Lafayette, IN, 47907-2100, USA.

Macromolecular Bioscience
|February 25, 2020
PubMed
Summary
This summary is machine-generated.

Elastin-like polypeptide (ELP) hydrogels show pH-responsive behavior. Acetylating ionizable residues like lysine and tyrosine significantly impacts their properties, making them promising for responsive applications.

Keywords:
elastomeric materialsenvironmentally responsive materialsionizable amino acidsrecombinant proteinssmart materials

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Materials Engineering

Background:

  • Protein-based hydrogels offer tunable properties for advanced applications.
  • Ionizable amino acids within hydrogels are key to achieving pH-responsiveness.
  • Elastin-like polypeptides (ELPs) provide a well-defined, crosslinkable platform for hydrogel development.

Purpose of the Study:

  • To investigate parameters influencing the pH-sensitive behavior of ELP hydrogels.
  • To characterize the mechanical and physical properties of ELP hydrogels across a pH range (3-11).
  • To assess the impact of crosslinker concentration, tag sequences, and residue acetylation on hydrogel performance.

Main Methods:

  • Fabrication of three distinct ELP-based crosslinked hydrogels.
  • Systematic variation of crosslinker content and ELP tag sequences.
  • Acetylation of lysine and tyrosine residues to block ionizable groups.
  • Characterization of hydrogel properties including stiffness, resilience, water content, swelling ratio, and zeta potential at varying pH levels.

Main Results:

  • Crosslinker concentration influenced hydrogel stiffness and resilience but not significantly water content, swelling, or pH sensitivity.
  • ELP tag sequences with varying histidine and aspartic acid content showed minimal impact on pH-sensitive properties.
  • Acetylation of lysine and tyrosine residues markedly reduced pH-sensitive zeta potential and altered low-pH behavior (reduced swelling and water content, increased stiffness).

Conclusions:

  • ELP hydrogels exhibit tunable pH-responsive characteristics.
  • The presence and accessibility of ionizable residues, particularly lysine and tyrosine, are critical for pH sensitivity.
  • Modified ELP hydrogels demonstrate potential for environmentally-responsive applications in drug delivery, tissue engineering, and microfluidics.