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Multiplexed Continuous Biosensing by Single-Molecule Encoded Nanoswitches.

Rafiq M Lubken^1,2, Arthur M de Jong^3,2, Menno W J Prins^1,3,2

¹Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven 5600 MB, The Netherlands.

|February 25, 2020

Summary

This study introduces a novel continuous biosensing method using single-molecule nanoswitches for real-time analysis. The technique enables multiplexed detection of oligonucleotide targets at picomolar concentrations in biological samples.

Keywords:

continuous biosensing kinetic identification multiplexing single-molecule biosensing

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Area of Science:

Bioanalytical Sciences
Nanotechnology
Molecular Biology

Background:

Single-molecule techniques offer high sensitivity but lack continuous monitoring capabilities.
Developing continuous biosensing methods is crucial for real-time bioanalysis.

Purpose of the Study:

To develop a multiplexed, continuous biosensing method using single-molecular nanoswitches.
To enable reliable quantification of single-molecule kinetics and target concentrations.

Main Methods:

Utilized analyte-sensitive, single-molecular nanoswitches with particle reporters.
Engineered reversible switching for binary state transitions.
Encoded multiplexing via nanoswitch dissociation and target concentration via association characteristics.

Main Results:

Demonstrated multiplexed, continuous monitoring of oligonucleotide targets.
Achieved detection at picomolar concentrations.
Validated the method in buffer and filtered human blood plasma via experiments and simulations.

Conclusions:

The developed nanoswitch method provides a robust platform for continuous and multiplexed biosensing.
This approach enhances the utility of single-molecule techniques for real-time bioanalytical applications.