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Published on: March 30, 2018
ABA-responsive ABRE-BINDING FACTOR3 activates DAM3 expression to promote bud dormancy in Asian pear
Qinsong Yang1,2,3, Bo Yang1,2,3, Jianzhao Li1,2,3
1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Abscisic acid (ABA) regulates pear bud dormancy by controlling DAM gene expression. Pear ABF3 activates PpyDAM3, while PpyABF2 modulates this, revealing a precise ABA-driven regulatory mechanism.
Area of Science:
- Plant Biology
- Molecular Biology
- Genetics
Background:
- Bud dormancy is crucial for perennial plant survival in cold climates.
- Abscisic acid (ABA) plays a key role in regulating bud endo-dormancy.
- Dormancy-associated MADS-box/SHORT VEGETATIVE PHASE-like (DAM/SVP) genes are downstream of ABA signaling but their regulation is unclear.
Purpose of the Study:
- To elucidate the regulatory mechanism of DAM/SVP gene expression in pear bud dormancy.
- To investigate the role of ABA and specific transcription factors in controlling PpyDAM3 expression.
- To understand how genetic variations in pear cultivars influence chilling requirements.
Main Methods:
- Confirmation of ABA's role in endo-dormancy and PpyDAM3 expression in pear buds.
- Correlation analysis between PpyABF3 expression and PpyDAM3 expression.
- Promoter binding assays to determine PpyABF3's interaction with the PpyDAM3 promoter.
- Transgenic experiments using pear calli to assess the function of PpyABF3 and PpyDAM3.
- Analysis of protein-protein interactions between PpyABF3 and PpyABF2.
Main Results:
- Pear bud dormancy and PpyDAM3 expression are controlled by ABA levels.
- Pear ABRE-BINDING FACTOR3 (PpyABF3) directly activates PpyDAM3 expression by binding to its promoter.
- Both PpyABF3 and PpyDAM3 inhibit cell division and growth in pear calli.
- PpyABF2 interacts with PpyABF3, disrupting PpyDAM3 promoter activation, thus precisely controlling DAM expression.
- Variations in the PpyDAM3 promoter may explain differences in chilling requirements among pear cultivars.
Conclusions:
- A finely tuned regulatory mechanism for ABA's effect on DAM gene expression in pear bud dormancy has been clarified.
- PpyABF3 acts as a positive regulator, while PpyABF2 acts as a negative modulator of PpyDAM3 expression.
- This study provides new insights into ABA-mediated bud dormancy regulation and its genetic basis in pears.
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