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Long noncoding RNA RHPN1-AS1 promotes colorectal cancer progression via targeting miR-7-5p/OGT axis

Wei Zheng1, Hui Li2, Hui Zhang1

  • 11Department of Gastrointestinal Surgery, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, 450003 Henan China.

Cancer Cell International
|February 27, 2020
PubMed
Abstract

Insights

Rhophilin Rho GTPase binding protein 1 antisense RNA 1 (RHPN1-AS1) promotes colorectal cancer (CRC) progression by regulating O-GlcNAcylation transferase (OGT) via sponging miR-7-5p. Silencing RHPN1-AS1 inhibits CRC growth, suggesting it as a potential therapeutic target.

Area of Science:

  • Molecular oncology
  • Genetics
  • Cancer biology

Background:

  • Rhophilin Rho GTPase binding protein 1 antisense RNA 1 (RHPN1-AS1) is an emerging oncogene implicated in various cancers.
  • Its specific role in colorectal cancer (CRC) pathogenesis was previously uncharacterized.

Purpose of the Study:

  • To elucidate the molecular function and regulatory mechanisms of RHPN1-AS1 in colorectal cancer progression.
  • To investigate RHPN1-AS1 as a potential therapeutic target for CRC.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) for RNA expression analysis.
  • Cell proliferation (CCK-8, EdU), apoptosis (flow cytometry), migration, and invasion assays in CRC cells.
  • Xenograft models for in vivo tumor growth assessment.
  • Western blot and mechanistic studies to explore gene interactions.

Main Results:

  • RHPN1-AS1 is significantly overexpressed in CRC cell lines and promotes proliferation, survival, migration, and invasion.
  • RHPN1-AS1 acts as a molecular sponge for miR-7-5p, leading to the upregulation of its downstream target, O-GlcNAcylation transferase (OGT).
  • STAT3 was identified as a transcription factor for RHPN1-AS1, and OGT overexpression reversed the anti-tumor effects of RHPN1-AS1 knockdown.

Conclusions:

  • RHPN1-AS1 promotes colorectal cancer progression by mediating OGT expression through sponging miR-7-5p.
  • RHPN1-AS1 represents a promising therapeutic target for colorectal cancer treatment.
Keywords:
Colorectal cancerOGTRHPN1-AS1miR-7-5p

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