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MIP-Based Impedimetric Sensor for Detecting Dengue Fever Biomarker.
Rabia Arshad1, Amina Rhouati2, Akhtar Hayat1
1Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS University Islamabad, Lahore Campus, Lahore, 54600, Pakistan.
Applied Biochemistry and Biotechnology
|February 27, 2020
Summary
This study presents a novel molecular imprinted polymer sensor for early dengue infection detection. The impedimetric sensor accurately identifies the non-structural protein 1 (NS1) biomarker in human serum samples.
Area of Science:
- Biomedical Engineering
- Electrochemistry
- Materials Science
Background:
- Dengue virus infection poses a significant global health challenge, necessitating early and accurate diagnostic methods.
- Non-structural protein 1 (NS1) is a critical and sensitive biomarker for early dengue virus detection.
- Existing diagnostic methods may have limitations in sensitivity, specificity, or turnaround time.
Purpose of the Study:
- To develop a highly sensitive and selective impedimetric sensor for the early detection of dengue infection.
- To utilize molecular imprinting technology for creating specific binding sites for the NS1 biomarker.
- To validate the sensor's performance in detecting NS1 in real human serum samples.
Main Methods:
- Fabrication of a molecular imprinted polymer (MIP) on a screen-printed carbon electrode (SPCE) using dopamine self-polymerization with NS1 as a template.
- Modification of SPCE with electrospun polysulfone (PS) nanofibers to enhance surface area and conductivity.
- Electrochemical characterization using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
- Optimization of sensor parameters and evaluation of detection range and limit of detection (LOD).
Main Results:
- The developed MIP-based impedimetric sensor demonstrated selective detection of the NS1 biomarker.
- Linear response observed in impedimetric measurements within the NS1 concentration range of 1 to 200 ng/mL.
- Achieved a low limit of detection (LOD) of 0.3 ng/mL for NS1.
- Successful determination of NS1 in human serum samples with high recovery rates (95-97.14%) and low standard deviations (<5%).
Conclusions:
- The developed MIP-based impedimetric sensor offers a promising platform for the early and accurate diagnosis of dengue infection.
- The sensor's ability to detect low concentrations of NS1 in complex matrices like human serum highlights its clinical potential.
- This technology could contribute to improved patient management and disease control strategies for dengue fever.
