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Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...

Protein-Protein Interfaces

Protein-Protein Interfaces

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Protein Networks

Protein Networks

Protein Organization

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....

Protein Organization

Protein Organization

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Updated: Dec 27, 2025

Mapping Dysfunctional Protein-Protein Interactions in Disease

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

Decoding Protein-protein Interactions: An Overview.

Olivia Slater¹, Bethany Miller¹, Maria Kontoyianni¹

¹Department of Pharmaceutical Sciences, Southern Illinois University, Edwardsville, IL 62026, United States.

Current Topics in Medicinal Chemistry

|February 27, 2020

Summary

This summary is machine-generated.

This review explores computational methods for predicting protein-protein interactions, crucial for drug discovery and repurposing. Understanding these interactions expands therapeutic targets beyond the "one drug, one target" model.

Keywords:

Protein-protein interactions binding site identification disease networks hot spots machine learning methods molecular recognition protein-protein docking.protein-protein interface

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Mapping Dysfunctional Protein-Protein Interactions in Disease

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Area of Science:

Biochemistry
Computational Biology
Drug Discovery

Background:

Traditional drug discovery relies on the "one drug, one target" paradigm.
Small molecules can interact with multiple targets, enabling drug repurposing.
Protein-protein interactions (PPIs) are increasingly important in drug discovery.

Purpose of the Study:

To review computational methods for predicting protein-protein complexes and interactions.
To highlight the role of PPIs in disease and drug discovery.
To present techniques for predicting interaction sites, hot spots, and binding sites.

Main Methods:

Computational methodologies for protein-protein docking.
Techniques for predicting protein-protein interactions and interfaces.
Approaches for identifying hot spots and binding sites.

Main Results:

Detailed description of computational methods for PPI prediction.
Discussion of networks in disease.
Presentation of a case study on chemokine CXC receptor 3 B-isoform.

Conclusions:

Computational prediction of PPIs is vital for expanding drug discovery targets.
Understanding PPI networks aids in disease mechanism elucidation.
Advanced computational tools facilitate the identification of novel therapeutic strategies.