Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

3.4K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.4K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.2K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.2K
Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered delivery systems for vaccine adjuvants.

International immunopharmacology·2026
Same author

A Microwave-Assisted, Rapidly Self-Healing, FFF-Printed TPU and Its Application in Supercritical Foaming.

Nanomaterials (Basel, Switzerland)·2026
Same author

All-Solid-State Bioinspired Nanofluidic Devices for Coupled Pressure- and Light-Driven Ionic Conversion.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Ion-sensitive in-situ gel loaded with rebamipide micelles for the treatment of diabetic keratopathy.

Colloids and surfaces. B, Biointerfaces·2026
Same author

α-Conotoxin LvID, an antagonist of α7 nicotinic acetylcholine receptor, mitigates Alzheimer-associated phenotypes by inhibiting Aβ deposition and reactive astrogliosis.

International journal of biological macromolecules·2026
Same author

Modified three-arm port configuration for single-position, single-docking robotic nephroureterectomy: perioperative and oncological outcomes.

Translational andrology and urology·2026
Same journal

An opposite pH-responsiveness "gating" strategy: Intelligent sporopollenin exine armor for targeted therapy of colitis.

Asian journal of pharmaceutical sciences·2026
Same journal

Photothermal-detonated functional macrophage membrane-camouflaged nano-crackers induce tumor cell wandering-anoikis.

Asian journal of pharmaceutical sciences·2026
Same journal

Multifunctional nanotherapeutics for targeted modulation of endoplasmic reticulum stress to potentiate cancer therapy.

Asian journal of pharmaceutical sciences·2026
Same journal

Red ginseng-derived nanovesicles to modulate osteoblast and osteoclastogenesis for osteoporosis therapy.

Asian journal of pharmaceutical sciences·2026
Same journal

Chimeric biohybrid nanovesicles induce immunogenic cell death for targeted and immune-potentiated glioblastoma therapy.

Asian journal of pharmaceutical sciences·2026
Same journal

Synergistic antimicrobial nanocomposite: Protamine-loaded PVP-capped silver nanoparticles for combating multidrug-resistant infections.

Asian journal of pharmaceutical sciences·2026
See all related articles

Related Experiment Video

Updated: Dec 27, 2025

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

25.9K

Exosome-based small RNA delivery: Progress and prospects.

Mei Lu1, Haonan Xing1, Zhe Xun2

  • 1School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.

Asian Journal of Pharmaceutical Sciences
|February 28, 2020
PubMed
Summary
This summary is machine-generated.

Exosomes show promise as natural nanocarriers for RNA interfering (RNAi) therapy, overcoming delivery challenges and reducing immunogenicity for effective gene silencing and disease treatment.

Keywords:
DeliveryExosomesNeurological disordersRNA interferingSmall RNAsTumors

More Related Videos

Author Spotlight: Development of a Large-Scale, Reproducible Production Method for Exosome Mimetics Using Magnetic Nanoparticles
05:36

Author Spotlight: Development of a Large-Scale, Reproducible Production Method for Exosome Mimetics Using Magnetic Nanoparticles

Published on: January 26, 2024

1.8K
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

32.3K

Related Experiment Videos

Last Updated: Dec 27, 2025

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

25.9K
Author Spotlight: Development of a Large-Scale, Reproducible Production Method for Exosome Mimetics Using Magnetic Nanoparticles
05:36

Author Spotlight: Development of a Large-Scale, Reproducible Production Method for Exosome Mimetics Using Magnetic Nanoparticles

Published on: January 26, 2024

1.8K
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

32.3K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • RNA interfering (RNAi) utilizes small RNAs for gene silencing, offering therapeutic potential for various diseases.
  • Current RNAi delivery methods face challenges including inefficiency and immunogenicity.
  • Exosomes, endogenous extracellular vesicles, are explored as natural nanocarriers for enhanced RNAi delivery.

Purpose of the Study:

  • To review the progress and challenges in RNAi therapy.
  • To discuss the potential of exosomes for small RNA delivery.
  • To highlight recent advances in exosome-based RNAi for disease treatment.

Main Methods:

  • Review of current literature on RNAi therapy and delivery vehicles.
  • Analysis of exosome biogenesis and function in intercellular communication.
  • Evaluation of exosome-based strategies for small RNA delivery.

Main Results:

  • Exosomes offer potential advantages over viral, synthetic polymer, and lipid-based carriers.
  • Exosomes exhibit higher delivery efficiency, lower immunogenicity, and better biocompatibility.
  • Exosomes possess intrinsic targeting capabilities, which can be enhanced with specific moieties.

Conclusions:

  • Exosomes represent a promising, natural alternative for delivering small RNAs in RNAi therapy.
  • Further research into exosome engineering and targeting can optimize their therapeutic application.
  • Exosome-based RNAi delivery holds significant potential for treating a wide range of diseases.