Periodic Membrane Potential and Ca²⁺ Oscillations in T Cells Forming an Immune Synapse

The immunological synapse (IS) is a specialized contact area formed between a T cell and an antigen presenting cell (APC). Besides molecules directly involved in antigen recognition such as the TCR/CD3 complex, ion channels important in the membrane potential and intracellular free Ca²⁺ concentration control of T cells are also recruited into the IS. These are the voltage-gated Kv1.3 and Ca²⁺-activated KCa3.1 K⁺ channels and the calcium release-activated Ca²⁺ channel (CRAC). However, the consequence of this recruitment on membrane potential and Ca²⁺ level control is not known. Here we demonstrate that the membrane potential (MP) of murine T cells conjugated with APCs in an IS shows characteristic oscillations. We found that depolarization of the membrane by current injection or by increased extracellular K⁺ concentration produced membrane potential oscillations (MPO) significantly more frequently in conjugated T cells than in lone T cells. Furthermore, oscillation of the free intracellular Ca²⁺ concentration could also be observed more frequently in cells forming an IS than in lone cells. We suggest that in the IS the special arrangement of channels and the constrained space between the interacting cells creates a favorable environment for these oscillations, which may enhance the signaling process leading to T cell activation.