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Related Concept Videos

Reporter Genes02:11

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Updated: Dec 27, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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HriGFP Novel Flourescent Protein: Expression and Applications.

Salma Saeed1, Hira Mehreen1, Umut Gerlevik2

  • 1Department of Biosciences, Comsats University Islamabad Campus, Islamabad, Pakistan.

Molecular Biotechnology
|February 29, 2020
PubMed
Summary

A novel fluorescent protein, HriGFP, effectively detects environmental pollutants like heavy metals and the pesticide methyl parathion. This biosensor offers a stable and sensitive method for monitoring water contamination.

Keywords:
BiosensorDockingFluorescent proteinsHriGFP

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Microbial biosensors offer cost-effective environmental pollutant monitoring.
  • Fluorescent proteins (fp) are versatile for developing small biosensing devices.
  • Naturally occurring fluorescent proteins exhibit stability across various conditions.

Purpose of the Study:

  • To explore the potential of a novel fluorescent protein, HriGFP from Hydnophora rigida, as a biosensor.
  • To evaluate HriGFP's efficacy in detecting environmental contaminants, specifically heavy metals and methyl parathion.
  • To assess HriGFP expression and biosensing capabilities in bacterial systems.

Main Methods:

  • Expression of HriGFP in bacterial systems (Gram-positive and Gram-negative).
  • Evaluation of HriGFP's biosensing capability in E. coli (BL21DE3) for heavy metals (Hg, Cu, As) and methyl parathion.
  • Fluorescence quenching analysis and structural/in-silico analysis to understand binding interactions.

Main Results:

  • HriGFP demonstrated discrete and stable expression in bacterial systems.
  • Significant fluorescent quenching of HriGFP was observed in the presence of heavy metals and methyl parathion.
  • Structural analysis confirmed heavy metal ion binding to HriGFP via amino acid residues; in-silico analysis revealed hydrogen bonding with methyl parathion.

Conclusions:

  • HriGFP functions as a sensitive and stable biosensor for detecting heavy metals and methyl parathion.
  • The study highlights HriGFP's potential for monitoring carcinogenic pesticides in water resources.
  • HriGFP offers a promising tool for detecting pollutants in industrial and agricultural water bodies.