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Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

Published on: November 28, 2016

Morphological and functional characterization of circulating hemocytes using microscopy techniques.

Sivakamavalli Jeyachandran^1,2, Kiyun Park¹, Ihn-Sil Kwak^1,3

¹Fisheries Science Institute, Chonnam National University, Yeosu, South Korea.

Microscopy Research and Technique

|February 29, 2020

Summary

This summary is machine-generated.

Microscopy revealed three hemocyte types in the mangrove crab *Episesarma tetragonum*, crucial for immune responses like phagocytosis and agglutination. These crab hemocytes also demonstrated significant antibacterial and antibiofilm activities.

Keywords:

Episesarma tetragonum agglutination agranulocytes cell responses confocal laser scanning microscopy granulocytes

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Area of Science:

Marine biology
Crustacean immunology
Cell biology

Background:

Hemocytes are vital circulating cells in invertebrates, mediating immune responses.
The mangrove crab *Episesarma tetragonum* serves as a model organism for studying cellular immunity.

Purpose of the Study:

To characterize the hemocytes of *Episesarma tetragonum* using microscopy.
To investigate the immune functions and antimicrobial activities of these hemocytes.

Main Methods:

Light microscopy with Giemsa, Ehrlich's, and Periodic acid-Schiff staining.
Assays for cell-mediated immune reactions (phagocytosis, agglutination) and antimicrobial/antibiofilm activity.
In situ microscopy for morphological analysis.

Main Results:

Three hemocyte types identified: granulocytes, semi-granulocytes, and hyalinocytes.
Hemocytes exhibited phagocytosis, agglutination (including yeast and human RBCs), and peroxidase-mediated adhesion.
Demonstrated significant antibacterial and antibiofilm activities, with biofilm inhibition visualized microscopically.

Conclusions:

*E. tetragonum* hemocytes possess diverse immune functions and antimicrobial properties.
These hemocytes are valuable for studying both cellular ultrastructure and functional immunity in invertebrates.