Rapid ¹³C NMR hyperpolarization delivered from para-hydrogen enables the low concentration detection and...

Monosaccharides, such as glucose and fructose, are important to life. In this work we highlight how the rapid delivery of improved ¹³C detectability for sugars by nuclear magnetic resonance (NMR) can be achieved using the para-hydrogen based NMR hyperpolarization method SABRE-Relay (Signal Amplification by Reversible Exchange-Relay). The significant ¹³C signal enhancements of 250 at a high field of 9.4 T, and 3100 at a low field of 1 T, enable the detection of trace amounts of these materials as well as the quantification of their tautomeric makeup. Using studies on ¹³C and ²H isotopically labelled agents we demonstrate how hyperpolarization lifetime (T ₁) values can be extended, and how singlet states with long lifetimes can be created. The precise quantification of d-glucose-¹³C₆-d ₇ at the millimolar concentration level is shown to be possible within minutes in conjunction with a linear hyperpolarized response as a function of concentration. In addition to the measurements using labelled materials, low concentration detection is also illustrated for millimolar samples with natural abundance ¹³C where isomeric form quantification can be achieved with a single transient.