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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Related Experiment Video

Updated: Dec 27, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

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Direct microRNA Sequencing Using Nanopore-Induced Phase-Shift Sequencing.

Jinyue Zhang1, Shuanghong Yan1, Le Chang1

  • 1State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China; Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.

Iscience
|March 1, 2020
PubMed
Summary
This summary is machine-generated.

Directly sequencing microRNAs (miRNAs) is now possible using Nanopore-induced phase-shift sequencing (NIPSS). This breakthrough offers single-molecule resolution for miRNA analysis, aiding in early disease diagnosis and cancer therapeutics.

Keywords:
Analytical ChemistryBiotechnologyMolecular BiologyNanotechnology

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • MicroRNAs (miRNAs) are crucial regulators of gene expression but are difficult to characterize due to low abundance and short length.
  • Existing miRNA detection methods lack single-molecule resolution, hindering detailed analysis.
  • Direct sequencing of single microRNAs has remained an unmet challenge in molecular biology.

Purpose of the Study:

  • To demonstrate the direct sequencing of microRNAs (miRNAs) at the single-molecule level.
  • To introduce Nanopore-induced phase-shift sequencing (NIPSS) as a novel method for miRNA analysis.
  • To overcome the limitations of current miRNA characterization techniques.

Main Methods:

  • Utilized Nanopore-induced phase-shift sequencing (NIPSS), a variant of nanopore sequencing.
  • Applied NIPSS to directly sequence short RNA molecules, including model microRNA sequences.
  • Validated the ability of NIPSS to distinguish between different miRNA identities, isoforms, and epigenetic variants.

Main Results:

  • Successfully demonstrated direct sequencing of microRNAs (miRNAs) with single-molecule resolution.
  • NIPSS effectively discriminated between various miRNA sequences, including isoforms and epigenetic modifications.
  • The technique proved capable of analyzing short RNA analytes, confirming its utility for miRNA analysis.

Conclusions:

  • Nanopore-induced phase-shift sequencing (NIPSS) enables direct, high-resolution sequencing of microRNAs (miRNAs).
  • This method complements existing miRNA sensing techniques by providing single-molecule insights.
  • Future development of NIPSS holds potential for early-stage disease diagnosis and novel cancer therapies.