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Related Concept Videos

Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass Analyzers: Overview01:13

Mass Analyzers: Overview

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The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
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Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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Microscope imaging mass spectrometry with a reflectron.

Robert J Burleigh1, Ang Guo1, Natasha Smith1

  • 1The Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom.

The Review of Scientific Instruments
|March 2, 2020
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Summary
This summary is machine-generated.

This study presents a novel time-of-flight microscope imaging mass spectrometer. It achieves high mass and spatial resolutions for detailed surface analysis, enabling high-throughput ion imaging.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Microscopy

Background:

  • Time-of-flight mass spectrometry (TOF-MS) is a powerful analytical technique.
  • Imaging mass spectrometry (IMS) provides spatial distribution of analytes.
  • Combining TOF-MS with IMS offers high sensitivity and spatial resolution.

Purpose of the Study:

  • To develop and characterize a time-of-flight microscope imaging mass spectrometer.
  • To achieve simultaneous high mass and spatial resolutions.
  • To demonstrate high-throughput ion imaging capabilities.

Main Methods:

  • Utilized a reflectron-equipped time-of-flight microscope imaging mass spectrometer.
  • Employed pulsed extraction differential acceleration ion optical focusing.
  • Implemented an event-triggered fast imaging sensor for ion image recording.

Main Results:

  • Achieved simultaneous mass resolution of 8100 ± 700 m/Δm and spatial resolution of 18 μm ± 6 μm.
  • Simulations indicate potential mass resolution up to 30 200 ± 2900 m/Δm.
  • Demonstrated high-throughput capability with event-triggered ion image recording.

Conclusions:

  • The developed TOF-IMS instrument offers excellent simultaneous mass and spatial resolutions.
  • The instrument's performance is primarily limited by detector response time.
  • Further optimization of extraction fields can enhance spatial resolution.
  • The system demonstrates potential for advanced surface analysis and high-throughput imaging applications.