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Intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging.

Yunzhao Wu, Yuqi Zhou, Chun-Jung Huang

|March 3, 2020

Summary

Frequency-shifted optofluidic time-stretch quantitative phase imaging (OTS-QPI) improves live cell analysis by simplifying phase retrieval. This advanced technique achieves high-accuracy leukemia cell classification and drug response evaluation.

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Area of Science:

Optofluidics
Quantitative Phase Imaging
Biomedical Optics

Background:

Optofluidic time-stretch quantitative phase imaging (OTS-QPI) offers high-throughput live cell analysis (>10,000 cells/s).
It decodes spectral interferograms carrying spatial cell profiles from microfluidic chips.
Current OTS-QPI faces challenges in phase retrieval, including unwrapping errors, high costs, and large data volumes.

Purpose of the Study:

To develop a frequency-shifted OTS-QPI method for simplified phase retrieval.
To enhance the utility of OTS-QPI for biological applications.
To demonstrate high-accuracy cell classification and drug evaluation using the improved technique.

Main Methods:

Proposed and experimentally demonstrated frequency-shifted OTS-QPI.
Shifted phase information to the baseband region for easier retrieval.
Utilized deep learning for image-based classification and drug evaluation.

Main Results:

Successfully brought phase information to the baseband region, overcoming high-frequency retrieval issues.
Achieved over 96% accuracy in image-based classification of leukemia cells.
Enabled effective evaluation of drug-treated leukemia cells.

Conclusions:

Frequency-shifted OTS-QPI effectively addresses limitations of conventional OTS-QPI.
The enhanced method boosts utility for high-throughput cell analysis and disease diagnostics.
This technique shows promise for accurate leukemia cell classification and drug response assessment.