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Updated: Dec 27, 2025
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Study of pathogenic factors of e.coli isolated from patients with peritonitis
Olha Yu Kosilova1, Oleksandra O Vovk1, Nataliia M Katelevska1
1Kharkiv National Medical University, Kharkiv, Ukraine.
Objective:
The aim: To study the biological properties of museum and clinical strains of E.coli isolated from patients with peritonitis.
Patients And Methods:
Materials and methods: It was used 94 strains (clinical, museum and reference). The ability of E. coli to adhere was investigated by hemadhezive method to formal human erythrocytes of 0 (I) Rh-positive blood group. The study measured the ability of microorganisms to produce gelatinaze, caseinase, fibrinolysin, hemolysin. To control of the enzyme activity the positive and negative control with reference strains were used. Synchronisation of cultures activity before experiments was achieved by one-time effect of low temperature (+4 С) during 30 minutes.
Results:
Results: To investigate the pathogenic factors of E. coli we carried out determining of proteolytic, gelatinous, caseinous, fibrinolytic, haemolytic and adhesion properties.
Conclusion:
Conclusions: In our investigation pathogen icspecies of Esherihies are virtually indistinguishable from representatives of normal microflora on its morphological, biochemical and cultural properties. During investigation of serological properties of selected of E. coli strains (n = 94) 65.8% of pathogenic serotypes were revealed. Moreover, all marked E. coli isolated from the abdominal cavity of children and adults, as well as museum strains related to enteropathogenic E. coli (O127: K63, O33: K-) and 1 - to enteroinvazive Escherichia coli (O144: K-).
Insights
This study compared museum and clinical Escherichia coli (E. coli) strains from peritonitis patients. Pathogenic serotypes were identified, with many strains linked to enteropathogenic or enteroinvasive E. coli.
Area of Science:
- Microbiology
- Clinical Bacteriology
Background:
- Escherichia coli (E. coli) is a common cause of peritonitis.
- Understanding the biological properties of clinical and museum strains is crucial for infection control and treatment.
Purpose of the Study:
- To investigate the biological properties of museum and clinical E. coli strains isolated from peritonitis patients.
- To identify pathogenic factors and serotypes within these E. coli strains.
Main Methods:
- Analyzed 94 E. coli strains (clinical, museum, reference).
- Assessed hemadhesion, gelatinase, caseinase, fibrinolysin, and hemolysin production.
- Utilized formal human erythrocytes and reference strains for controls.
Main Results:
- Determined proteolytic, gelatinous, caseinous, fibrinolytic, hemolytic, and adhesion properties.
- Identified 65.8% of pathogenic serotypes among the E. coli strains.
- Classified strains as enteropathogenic (e.g., O127:K63) or enteroinvasive (e.g., O144:K-).
Conclusions:
- Pathogenic E. coli strains showed minimal differences in morphological, biochemical, and cultural properties compared to normal flora.
- Clinical and museum strains frequently exhibited pathogenic characteristics, including serotypes associated with severe infections.
- The findings highlight the importance of characterizing E. coli strains for effective clinical management.
