Related Experiment Video
Updated: Oct 14, 2025
Author Spotlight: Exploring the Antibacterial Effects of Zinc Oxide Nanoparticles in Overcoming Antibiotic Resistance
Published on: September 27, 2024
STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY
Evheniia A Shtaniuk1, Oleksandra O Vovk1, Larisa V Krasnikova1
1KHARKIV NATIONAL MEDICAL UNIVERSITY, KHARKIV, UKRAINE.
Objective:
The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them.
Patients And Methods:
Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method ("well" method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis.
Results:
Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine.
Conclusion:
Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro.
Insights
This study evaluated antibacterial agents, including dioxidine and levofloxacin, against common surgical wound pathogens. Preparations with dioxidine and levofloxacin showed significant effectiveness in inhibiting bacterial growth in vitro.
Area of Science:
- Microbiology
- Pharmacology
- Infectious Diseases
Background:
- Surgical site infections pose a significant clinical challenge.
- Identifying effective antimicrobial agents is crucial for managing these infections.
- Standard bacterial strains like S. aureus, E. coli, and P. aeruginosa are common pathogens.
Purpose of the Study:
- To assess the in vitro antibacterial activity of preparations containing dioxidine and levofloxacin.
- To compare the efficacy of different formulations against key pathogens.
- To determine the most effective agents for purulent-inflammatory processes in surgical wounds.
Main Methods:
- Utilized standard strains: S. aureus ATCC 25923, E. coli ATCC 25922, P. aeruginosa ATCC 27853.
- Employed the disco-diffusion method on Mueller-Hinton agar for sensitivity testing.
- Agar diffusion method ('well' method) was used to measure antibacterial activity of solutions and ointments.
Main Results:
- All tested antibacterial preparations demonstrated effectiveness against S. aureus, E. coli, and P. aeruginosa.
- Dioxidine with decamethaxin solution and levofloxacin with decamethaxin ointment showed larger inhibition zones against S. aureus and P. aeruginosa.
- E. coli strains were most susceptible to Dioxisole solution and dioxidine ointment.
Conclusions:
- The evaluated antibacterial preparations are effective against tested bacterial strains.
- Most strains exhibited high sensitivity to the tested agents in vitro.
- Specific formulations showed superior efficacy against particular pathogens.
More Related Videos
06:18A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
09:26Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
Published on: January 22, 2021
Related Concept Videos
01:28Antimicrobial Effectiveness
01:28Gene Regulation in Microbial Communities: Quorum Sensing
01:23Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
01:27Chemical Agents for Microbial Control
01:27Combined Effects of Drugs: Synergism
Such synergistic combinations...
01:18Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...