Nanostructured nickel oxide electrodes for non-enzymatic electrochemical glucose sensing

Nanostructured nickel (Ni) and nickel oxide (NiO) electrodes were fabricated on Ni foils using the glancing angle deposition (GLAD) technique. Cyclic voltammetry and amperometry showed the electrodes enable non-enzymatic electrochemical determination of glucose in strongly alkaline media. Under optimized conditions of NaOH concentration and working potential (~ 0.50 V vs. Ag/AgCl), the GLAD electrodes performed far better than bare Ni foil electrodes, with the GLAD NiO electrode showing an outstanding sensitivity (4400 μA mM^-1 cm^-2), superior detection limit (7 nM), and wide dynamic range (0.5 μM-9 mM), with desirable selectivity and reproducibility. Based on their performance at a low concentration, the GLAD NiO electrodes were also used to quantify glucose in artificial urine and sweat samples which have significantly lower glucose levels than blood. The GLAD NiO electrodes showed negligible response to the common interferents in glucose measurement (uric acid, dopamine, serotonin, and ascorbic acid), and they were not poisoned by high amounts of sodium chloride. Graphical abstract The figures depict (A) SEM image of vertical post-GLAD NiO electrodes used for non-enzymatic electrochemical glucose monitoring, and (B) calibration plots of the three different electrodes.