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Updated: Dec 27, 2025

Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Control of Akt activity and substrate phosphorylation in cells
1Department of Structural and Computational Biology, University of Vienna, Vienna, Austria.
Abstract:
Protein kinase B/Akt is a serine/threonine kinase that links receptors coupled to the PI3K lipid kinase to cellular anabolic pathways. Its activity in cells is controlled by reversible phosphorylation and an intramolecular lipid-controlled allosteric switch. In this review, I outline the current progress in understanding Akt regulatory mechanisms, define three models of Akt activation in cells, and highlight how intramolecular allosterism cooperates with cell-autonomous mechanisms to control Akt localization and activity and direct it toward specific sets of substrates in cells.
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