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A ratiometric 19F MR-based method for the quantification of Ca2+ using responsive paramagnetic probes.
Giuseppe Gambino1, Tanja Gambino, Rolf Pohmann
1MR Neuroimaging Agents, MPI for Biological Cybernetics, Tuebingen, Germany. goran.angelovski@tuebingen.mpg.de.
Chemical Communications (Cambridge, England)
|March 5, 2020
Summary
Researchers developed a novel fluorine-19 (19F) MRI method to measure extracellular calcium concentration. A new calcium-sensing 19F MRI probe shows significant signal changes upon binding calcium ions.
Area of Science:
- Biomedical imaging
- Nuclear magnetic resonance (NMR) spectroscopy
- Inorganic chemistry
Background:
- Extracellular calcium (Ca2+) is crucial for physiological processes.
- Accurate measurement of extracellular Ca2+ is challenging.
- Current methods have limitations in spatial and temporal resolution.
Purpose of the Study:
- To develop a novel method for assessing extracellular calcium concentration.
- To introduce a new calcium-responsive 19F MRI probe.
- To evaluate the performance of the developed probe and method.
Main Methods:
- Utilized fluorine-19 (19F) chemical shift imaging.
- Developed a custom-made, lanthanide-based 19F MRI probe.
- The probe exhibits signal modulation upon calcium ion coordination.
Main Results:
- Demonstrated successful assessment of extracellular calcium concentration using 19F MRI.
- The developed probe showed a strong and specific response to calcium ions.
- Characterized the probe's performance in calcium-sensing applications.
Conclusions:
- The presented 19F MRI method offers a promising approach for measuring extracellular calcium.
- The novel calcium-responsive probe enables sensitive and specific detection of calcium ions.
- This technique has potential applications in various physiological and pathological studies.
