Photoelectro-Enzymatic Glucose Reusable Biosensor by Using Dithienylethene Mediators.

Area of Science:

• Electrochemistry
• Photochemistry
• Biosensor technology

Background:

• Traditional colorimetric biosensors often use electrochromic mediators with limited recyclability.
• The regeneration of these mediators is complex, often requiring electrochemical methods or antioxidants, hindering disposable designs.

Purpose of the Study:

• To introduce a novel photoelectrochromic compound, diacid dithienylethene (DTE), as a reusable substrate for enzymatic biosensing.
• To demonstrate light-induced control over enzymatic substrate availability and biosensor regeneration.

Main Methods:

• Utilized a diacid dithienylethene (DTE) photoelectrochromic compound as a substrate for horseradish peroxidase (HRP).
• Investigated the photoisomerization of DTE between its open (DTEo) and closed (DTEc) forms.
• Employed a glucose oxidase (GOx)-HRP cascade system for light-induced glucose detection.

Main Results:

• Demonstrated light-induced enzymatic detection of glucose using the DTE/HRP system.
• Showcased the ability to control substrate availability and regenerate the biosensor through light pulses.
• Achieved multiple cycles of biosensor use and regeneration.

Conclusions:

• Presented the first use of a photoelectrochromic compound (DTE) for light-controlled, reusable biosensing.
• Highlighted the potential for developing advanced photonic biosensing systems with light-induced regeneration.
• Overcame limitations of traditional disposable biosensors through a novel light-controlled approach.