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Updated: Dec 27, 2025

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
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PX Domain-Containing Kinesin KIF16B and Microtubule-Dependent Intracellular Movements

Bo-Jie Li1, Hao Chen2, Su-Su Jiang1

  • 1Department of Biochemistry & Molecular Biology, Medical College, University of South China, Hengyang, 421001, Hunan, China.

The Journal of Membrane Biology
|March 7, 2020
PubMed

Insights

Kinesin family member 16B (KIF16B) transports cellular cargo and forms endosomal tubules. This review explores KIF16B

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Kinesin family member 16B (KIF16B) is a kinesin-3 motor protein.
  • It possesses a PhoX homology (PX) domain that binds to PI(3)P-rich membranes.
  • KIF16B moves towards microtubule plus ends, regulated by its stalk region's coiled coils.

Purpose of the Study:

  • To review recent advances in KIF16B's structural and physiological characteristics.
  • To summarize diseases associated with KIF16B dysfunction.
  • To speculate on KIF16B's role in intracellular cholesterol trafficking.

Main Methods:

  • Literature review of structural and physiological studies on KIF16B.
  • Analysis of clinical data linking KIF16B to disease processes.
  • Integration of findings on Rab protein regulation of KIF16B-mediated transport.

Main Results:

  • KIF16B is crucial for intracellular cargo transport and endosomal tubule formation.
  • Rab proteins regulate KIF16B-mediated vesicle trafficking.
  • KIF16B dysfunction is implicated in intellectual disability, lipid metabolism disorders, and brain tumor metastasis.

Conclusions:

  • KIF16B plays a significant role in membrane transport and cellular organization.
  • Disruptions in KIF16B function are linked to various pathological conditions.
  • KIF16B may act as a key adaptor in intracellular cholesterol transport pathways.
Keywords:
Cholesterol transportEndocytic traffickingKIF16BKinesin

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