Related Experiment Video
Updated: Dec 26, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
High Contiguity De Novo Genome Sequence Assembly of Trifoliate Yam (Dioscorea dumetorum) Using Long Read Sequencing
Christian Siadjeu1,2, Boas Pucker2,3, Prisca Viehöver2
1Institute for Biology and Environmental Sciences, Biodiversity and Evolution of Plants, Carl-von-Ossietzky University Oldenburg, Carl-von-Ossietzky Str. 9-11, 26111 Oldenburg, Germany.
Abstract:
Trifoliate yam (Dioscorea dumetorum) is one example of an orphan crop, not traded internationally. Post-harvest hardening of the tubers of this species starts within 24 h after harvesting and renders the tubers inedible. Genomic resources are required for D. dumetorum to improve breeding for non-hardening varieties as well as for other traits. We sequenced the D. dumetorum genome and generated the corresponding annotation. The two haplophases of this highly heterozygous genome were separated to a large extent. The assembly represents 485 Mbp of the genome with an N50 of over 3.2 Mbp. A total of 35,269 protein-encoding gene models as well as 9941 non-coding RNA genes were predicted, and functional annotations were assigned.
Related Concept Videos
Genome Annotation and Assembly
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Sanger Sequencing
Oligosaccharide Assembly
Multiple sugar molecules that may or may...

