Calcium-binding lysozymes

It was found that pigeon lysozyme binds one calcium ion, as does equine lysozyme. The protein was eluted with equimolar calcium ions from a Bio-Gel P-60 column. The binding constants of equine and pigeon lysozymes were determined to be 2 x 10(6) and 1.6 x 10(7) M-1, respectively, in 0.1 M KCl at pH 7.1 and 20 degrees C. During evolution the gene of calcium-binding lysozyme is deduced to be separated from that of non-calcium-binding lysozyme by gene duplication before splitting of avian and mammalian lineages, from their amino-acid sequences. It is assumed that the alpha-lactalbumin might have evolved from calcium-binding lysozyme.