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Updated: Aug 10, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
The structures of the two lipopolysaccharide O-chains produced by Salmonella boecker
1Division of Biological Sciences, National Research Council of Canada, Ottawa, Ont.
Abstract:
Salmonella boecker, which belongs to group 0:6, 14(H) and shows the antigenic factors 6, 14, [1], and [25], defined by the Kauffmann-White system, produces two lipopolysaccharides differing from each other in the structures of their 0-poly-saccharide moieties. By glycose composition, partial hydrolysis, nitrous acid deamination, methylation, optical rotation, and 1H and 13C nuclear magnetic resonance studies, the O-polysaccharides were demonstrated to be high-molecular-weight polymers (I and II) composed of either structurally related repeating tetrasaccharide or repeating pentasaccharide units having the structures and (table; see text).
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