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Protocols for Innate Lymphoid Cell Phenotypic and Functional Characterization: An Overview.
1Laboratory of T Cell Regulation, Institute of Cellular Medicine, Newcastle University, Newcastle Upon Tyne, UK. shoba.amarnath@newcastle.ac.uk.
Methods in Molecular Biology (Clifton, N.J.)
|March 10, 2020
Summary
Innate lymphoid cells (ILCs) are crucial for early immune defense at barrier surfaces. This book provides detailed protocols for studying ILC development, characterization, and function in mice and humans.
Area of Science:
- Immunology
- Cell Biology
Background:
- The innate immune system provides a rapid, first line of defense against pathogens.
- Innate lymphoid cells (ILCs) are a recently discovered family of immune cells that lack antigen receptors but produce T cell-associated cytokines.
- ILCs are critical for immunity at barrier surfaces and were previously overlooked in favor of myeloid cells.
Purpose of the Study:
- To provide comprehensive protocols for the study of innate lymphoid cells (ILCs).
- To detail methods for analyzing ILC development, characterization, and function.
- To facilitate research on ILCs in both mouse and human systems.
Main Methods:
- Detailed laboratory protocols for ILC research.
- Techniques for delineating ILC-specific functions.
- Methods applicable to both mouse and human ILC studies.
Main Results:
- Established protocols for studying ILCs are presented.
- The complexity of ILC phenotype and function is addressed.
- Methods enable differentiation of ILCs from other immune cells.
Conclusions:
- ILCs are vital regulators of barrier immunity.
- Advanced protocols are necessary for accurate ILC research.
- This resource aids in understanding ILC roles in health and disease.
