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Updated: Dec 26, 2025
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Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
Published on: March 23, 2022
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Isolation, Culture, and Induction of Plasticity in ILC2s
Jonathan Silver1, Alison A Humbles2, Yoichiro Ohne3
1AstraZeneca, Gaithersburg, MD, USA. jonathan.silver@astrazeneca.com.
Methods in Molecular Biology (Clifton, N.J.)
|March 10, 2020
Summary
Protocols for maintaining, expanding, and inducing plasticity in innate lymphoid cells type 2 (ILC2s) are presented. These methods enable further study of ILC2 function and biology in various contexts.
Area of Science:
- Immunology
- Cell Biology
Background:
- Innate lymphoid cells (ILCs) are crucial immune cells found in mucosal tissues.
- ILCs are classified into subsets (ILC1, ILC2, ILC3) with distinct functions.
- ILC subsets display heterogeneity and plasticity, influencing immune responses.
Purpose of the Study:
- To establish protocols for the maintenance and expansion of ILC2s.
- To develop methods for inducing plasticity in ILC2s.
- To facilitate molecular interrogation of ILC function and biology.
Main Methods:
- Described protocols for culturing mouse and human ILC2s.
- Detailed methods for inducing phenotypic and functional plasticity in ILC2s.
- Established conditions for both in vitro and in vivo studies.
Main Results:
- Successfully maintained and expanded mouse and human ILC2 populations.
- Demonstrated the ability to induce plasticity in ILC2s under specific conditions.
- Generated ILC2 cells suitable for detailed molecular analysis.
Conclusions:
- The developed protocols provide a robust platform for ILC2 research.
- These methods allow for the investigation of ILC2 plasticity and its functional consequences.
- The study enhances our understanding of ILC2s in immunity and homeostasis.
