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Updated: Dec 26, 2025
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
Published on: April 13, 2015
Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment
Grace Mallett1, Warren Patterson1, Megan Payne1
1Laboratory of T cell Regulation, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Abstract:
Innate lymphoid cells (ILCs) are important for both tissue immunity and tissue homeostasis. They are classified into three groups: Group 1 ILCs include NK cells, which are important in eliciting immunity against intracellular pathogens; group 2 ILCs protect against parasitic helminths; and group 3 ILCs protect against extracellular pathogens. The role of ILCs in cancer immunity remains unclear. In this chapter, we discuss methods for isolating and characterizing tumor-infiltrating ILC subsets within the tumor microenvironment in an experimental murine model of B16 melanoma. The chapter also highlights the expression of PD-1 on the various ILC subsets within the tumor microenvironment.
