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Updated: Dec 26, 2025
Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Published on: May 21, 2012
Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease.
Omer S Omer1, Nick Powell2, Graham M Lord3,4
1Peter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, King's College London, London, UK.
Innate lymphoid cells (ILCs) are key to gut immunity and homeostasis. This study details methods to analyze ILCs and their cytokine production in inflammatory bowel disease (IBD) research.
Area of Science:
- Immunology
- Gastroenterology
Background:
- Innate lymphoid cells (ILCs) are crucial for innate immunity and intestinal homeostasis.
- ILCs share similarities with CD4+ T helper cells and are implicated in inflammatory bowel disease (IBD).
Purpose of the Study:
- To outline protocols for characterizing human ILCs and their intracellular cytokine expression.
- To provide methods for isolating immune cells from human blood and colon for IBD studies.
Main Methods:
- Flow cytometry for ILC characterization and intracellular cytokine analysis.
- Protocols for isolating peripheral blood mononuclear cells (PBMCs).
- Protocols for isolating colonic lamina propria mononuclear cells (LPMCs).
Main Results:
- Established methods for analyzing ILC phenotype and function.
- Demonstrated isolation techniques for relevant human immune cell populations.
Conclusions:
- The described protocols facilitate the study of ILCs in human IBD.
- These methods are essential for understanding ILC roles in intestinal inflammation.
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