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Updated: Dec 26, 2025

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
Published on: May 21, 2012
Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease
Omer S Omer1, Nick Powell2, Graham M Lord3,4
1Peter Gorer Department of Immunobiology, School of Immunology & Microbial Sciences, King's College London, London, UK.
Abstract:
Innate lymphoid cells (ILCs) are emerging as important effectors of innate immunity and play a critical role in maintaining intestinal immune homeostasis. They are tissue-residing immune cells that can be subdivided based on master transcription factor and cytokine expression, bearing striking resemblance to their CD4+ T helper (Th) cell counterparts. ILCs are increasingly recognized as potential mediators of inflammatory bowel disease (IBD) providing a need to explore their functional and phenotypic differences in health vs. disease. In this chapter we outline protocols for the characterization of human ILCs and intracellular cytokine expression using flow cytometry. We include protocols for isolating human peripheral blood and colonic lamina propria mononuclear cells essential for evaluating human IBD specimens.
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