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Updated: Dec 26, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
[Progress in the application of single molecular sequencing in tumor diagnosis]
Jie Sheng1, Yuxin Wang1, Jiangfa Qi1
1Institute of Biology and Medicine, College of Life Science and Health, Wuhan University of Science and Technology, Wuhan 430081, Hubei, China.
Abstract:
Tumor development is usually related to the genetic mutation and abnormal expression of multiple genes. Comprehensive analysis of tumor genome, transcriptome and epigenetics is very important for the rapid identification of disease-specific gene clusters and modification sites. Previously, the next-generation sequencing technology was mainly used to explore the information of genomes, however, it cannot meet the requirement of mechanical researches due to several problems such as difficult sequence assembly and leak detection of the low abundance factors. Therefore, single molecule sequencing technology gradually emerged with its unique superiority. This paper reviews the research processes of single molecule sequencing technology in several human tumors, and prospecs its application in clinical diagnosis.
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