Dispersion quality of single-walled carbon nanotubes reveals the recognition sequence of DNA

The specific recognition between DNA and single-walled carbon nanotubes (SWCNTs) has enabled wide applications, especially in the chiral sorting of SWCNTs. However, the molecular recognition mechanism has not been fully understood. In our work, various DNA-SWCNT dispersions were prepared by the ultrasonic dispersion method, and characterized by UV absorption spectroscopy, fluorescence emission spectroscopy, zeta potential measurement, SDBS exchange kinetics and computer simulation. The effect of DNA sequence on the structure and properties of hybrid molecules was analyzed. Data analysis showed that DNA with specific recognition had better dispersion quality of the corresponding SWCNT, which means that higher content of monodispersed SWCNTs was obtained. The high-quality dispersion of the DNA-SWCNT pair was attributed to the stronger binding between DNA and SWCNT, resulting in a tighter conformation of DNA on the SWCNT surface and a larger zeta potential of DNA-SWCNT hybrids. Consequently, DNA-SWCNT dispersions of the recognition pair exhibited better stability against salt and stronger fluorescence emission intensity. However, the correlation between specific recognition and DNA coverage on SWCNT was not observed. This work gives more insight into the recognition mechanism between DNA and SWCNTs.