Aptamer-functionalized chitosan magnetic nanoparticles as a novel adsorbent for selective extraction of ochratoxin A

The preparation and application of aptamer-functionalized chitosan magnetic nanoparticles (Fe₃O₄@CTS@Apt nanoparticles) for selective extraction and determination ochratoxin A (OTA) were described in this study. Magnetic nanoparticle was synthesized by the coprecipitation method followed by coating with chitosan to improve its stability and biocompatibility. Further characterization was performed by scan electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and magnetic property measurement, and the results clearly indicated that the obtained magnetic chitosan nanoparticle was composed of magnetic core and chitosan coating layer. Aptamers specific to OTA were coupled onto the magnetic chitosan nanoparticles, and an extraction procedure was developed by optimization. When challenged with food samples fortified with OTA at 5 and 10 μg/kg, recoveries ranging from 91.3% to 99.1% with relative standard deviation (RSD) ≤ 4.2% were achieved by aptamer-functionalized magnetic extraction, which is very close to the results obtained by immunoaffinity chromatography extraction, indicating that this magnetic adsorbent could be hopefully used to achieve a fast and efficient extraction and detection of OTA in food samples.