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AS-LAMP: A New and Alternative Method for Genotyping.

Pooria Gill¹, Arash Hadian Amree²

¹Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

Avicenna Journal of Medical Biotechnology

|March 11, 2020

Summary

Loop-mediated isothermal amplification (LAMP) is a reliable method for detecting pathogens. This review shows LAMP is also a viable alternative technique for genotyping Single Nucleotide Polymorphisms (SNPs).

Keywords:

AS-LAMP Genotyping LAMP Single nucleotide polymorphisms

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Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Various methods exist for genotyping Single Nucleotide Polymorphisms (SNPs) and mutations in nucleic acid sequences, with applications in agriculture and medicine.
The Loop-mediated isothermal amplification (LAMP) method, initially developed for pathogen detection, is an isothermal DNA amplification technique.

Purpose of the Study:

To review studies utilizing the LAMP method for Single Nucleotide Polymorphism (SNP) detection.
To evaluate the reliability and potential of LAMP as an alternative genotyping technique.

Main Methods:

Literature review of studies employing LAMP for SNP detection.
LAMP utilizes four unique primers targeting six distinct gene regions for isothermal DNA amplification via strand displacement.

Main Results:

The LAMP technique demonstrated reliability in various studies for SNP detection.
The amplification and detection of target DNA sequences can be achieved in a single step.

Conclusions:

The LAMP method is a promising and reliable alternative for genotyping SNPs.
Further research is recommended to explore and validate the application of LAMP for genotyping.