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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

62.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Dec 26, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies.

Rashid Aman1, Ahmed Mahas1, Magdy Mahfouz1

  • 1Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.

ACS Synthetic Biology
|March 12, 2020
PubMed
Summary
This summary is machine-generated.

CRISPR-based biosensors offer rapid, ultrasensitive pathogen detection for infectious diseases, overcoming limitations of traditional methods. These advanced nucleic acid detection technologies enable point-of-care diagnostics, even in resource-limited settings.

Keywords:
CRISPR-Casbiosensing platformsdiagnosisnucleic acid detection

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Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Biosensing Technologies

Background:

  • Accurate pathogen identification is crucial for infectious disease management, yet current diagnostic methods face challenges, especially in resource-limited areas.
  • Conventional nucleic acid detection methods like PCR are effective but require expensive equipment and skilled personnel.
  • CRISPR/Cas systems offer precise DNA/RNA recognition and cleavage, with collateral activities suitable for sensitive biosensing.

Purpose of the Study:

  • To review recent advances in CRISPR-based biosensing technologies for pathogen detection.
  • To highlight the potential of CRISPR systems for rapid, accurate, and ultrasensitive diagnostics.
  • To explore the application of CRISPR-based sensors in various fields, including human health and agriculture.

Main Methods:

  • Utilizing CRISPR/Cas systems (e.g., Cas13, Cas12a, Cas14) that exhibit collateral catalytic activity upon target recognition.
  • Employing collateral activity for nucleic acid detection, such as signal generation via labeled nucleic acid degradation.
  • Integrating CRISPR/Cas systems with lateral flow systems for point-of-care diagnostic devices.

Main Results:

  • CRISPR-based biosensors can achieve attomolar sensitivity for multiplexed detection of multiple targets.
  • These systems demonstrate potential for inexpensive, accurate, and highly sensitive in-field diagnostics.
  • CRISPR technology enables development of novel sensors with broad applications.

Conclusions:

  • CRISPR-based biosensing represents a significant advancement in nucleic acid detection technologies.
  • These systems offer a promising alternative to traditional diagnostic methods, particularly for resource-limited settings.
  • CRISPR biosensors have vast potential for diverse applications, revolutionizing diagnostics.