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Morphological changes after acetylcholinesterase (AChE) inhibition by dichlorvos (DDVP) in young rabbit brain
1Laboratory of Developmental Neuropathology, Medical Research Centre, Polish Academy of Sciences, Warsaw.
Abstract:
The present study is concerned with the lesions of nerve- and glial cells after AChE inhibition during brain development. The investigations were performed on rabbits. Dichlorvos (DDVP) an known inhibitor of AChE was given to young animals per os (4-8 mg/kg of body weight) from 6th to 15th day of life. Cerebral and cerebellar cortex and corpus callosum were examined in electron microscope. Our investigations revealed pathological changes in all neuroectodermal elements. It seems that inhibition of AChE induced by DDVP intoxication led to damage of biologic membranes. This is particularly harmful for differentiating and maturing cells.
Insights
Dichlorvos (DDVP) exposure during early brain development caused nerve and glial cell damage in rabbits. This acetylcholinesterase (AChE) inhibitor harms developing brain cells by damaging biologic membranes.
Area of Science:
- Neuroscience
- Developmental Biology
- Toxicology
Background:
- Acetylcholinesterase (AChE) is crucial for neuronal function.
- Developmental exposure to toxins can have lasting neurological effects.
- Organophosphate pesticides, like Dichlorvos (DDVP), are known AChE inhibitors.
Purpose of the Study:
- To investigate the effects of AChE inhibition on nerve and glial cells during brain development.
- To examine the cellular damage caused by Dichlorvos (DDVP) exposure in young rabbits.
Main Methods:
- Young rabbits were administered Dichlorvos (DDVP) orally from day 6 to 15 of life.
- Dose administered: 4-8 mg/kg body weight.
- Cerebral cortex, cerebellar cortex, and corpus callosum were analyzed using electron microscopy.
Main Results:
- Pathological changes were observed in all neuroectodermal elements examined.
- Dichlorvos (DDVP) intoxication led to damage of biologic membranes.
- Developing and maturing nerve and glial cells were particularly vulnerable.
Conclusions:
- AChE inhibition by Dichlorvos (DDVP) during brain development causes significant cellular damage.
- The observed membrane damage is detrimental to differentiating and maturing neural cells.
- Early-life exposure to AChE inhibitors poses a risk to neurodevelopment.