Multiplexing of Immune Markers via Electrochemiluminescence Immunoassays for Systems Biology

Electrochemiluminescence immunoassays are based on the principle of light emission in a chemical environment to detect and analyze different proteins and biomolecules. It has numerous advantages over traditional analytical methods including conservation of sample, high sensitivity, broad range, and relative ease of use. Herein, we describe the electrochemiluminescence methods by using Mesoscale Discovery System with recommendations and optimization of protocols to aid in discovery of biological relevant markers and also discuss avoidance of major pitfalls for accurate biomarker detection.