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Updated: Dec 26, 2025

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Reproducibility of Heart Rate Variability Indices at Post-maximal Exercise
Jaqueline Alves Araújo1, Tiago Peçanha2, Fabiula Isoton Novelli1
1Physical Education, Universidade Federal de Mato Grosso, Cuiabá, Brazil.
Heart rate variability (HRV) measurements are reproducible after maximal exercise in untrained men. Time-domain HRV indices demonstrated higher reliability than frequency-domain indices, irrespective of recovery type.
Area of Science:
- Exercise Physiology
- Cardiovascular Health
- Sports Science
Background:
- Heart rate variability (HRV) is a key indicator of autonomic nervous system function.
- Assessing HRV reproducibility after maximal exercise is crucial for accurate physiological monitoring.
- Understanding recovery's impact on HRV reliability is essential for exercise prescription and interpretation.
Purpose of the Study:
- To evaluate the reproducibility of time-domain and frequency-domain HRV indices following maximal exercise.
- To compare the reliability of HRV measurements between passive and active recovery protocols.
- To determine if HRV indices analyzed in short segments are reproducible after strenuous physical exertion.
Main Methods:
- Eleven healthy, untrained men underwent four incremental exercise tests to exhaustion.
- Heart rate variability was recorded during passive and active recovery periods.
- Key HRV indices, including LnRMSSD and LnSDNN, were analyzed in 30-second segments and 5-10 minute intervals.
Main Results:
- High reliability (ICC: 0.72-0.96) and good agreement were observed for LnRMSSD and LnSDNN in both recovery types.
- Time-domain HRV indices (5-10 min) showed higher reliability (ICC: 0.59-0.88) and better agreement (CV: 7.67-16.26%) than frequency-domain indices.
- Reproducibility was satisfactory for both time-domain and frequency-domain indices, with time-domain measures being more reliable.
Conclusions:
- Time-domain heart rate variability indices demonstrate satisfactory reproducibility after maximal exercise in untrained men.
- Recovery type (passive vs. active) did not significantly impact the reproducibility of most HRV indices.
- Time-domain HRV measures are more reliable than frequency-domain measures for assessing physiological responses post-maximal exercise.
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