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Simulated Moving Bed Purification for Flavonoids from Tartary Buckwheat Shell
Liangyu Li1,2, Wanxia Liu3, Dawei Song3
1National Coarse Cereal Engineering Technology Research Center, Heilongjiang Bayi Agricultural University, No.5, Xinfeng Road high tech District Daqing 163319, PR China.
Journal of Chromatographic Science
|March 13, 2020
Summary
This study successfully purified Tartary buckwheat shell flavonoids (TBSF) using simulated moving bed (SMB) chromatography, achieving high purity and yield. The purified TBSF demonstrated significant antioxidant and α-glucosidase inhibitory activities, suggesting SMB
Area of Science:
- Biotechnology
- Natural Product Chemistry
- Separation Science
Background:
- Tartary buckwheat shells are a rich source of flavonoids with antioxidant properties.
- Current purification methods for Tartary buckwheat shell flavonoids (TBSF) are limited to laboratory scale, hindering industrialization.
- Advanced separation technologies are needed for efficient and scalable TBSF purification.
Purpose of the Study:
- To develop and optimize a scalable purification method for Tartary buckwheat shell flavonoids (TBSF).
- To evaluate the efficacy of simulated moving bed (SMB) chromatography for industrial TBSF production.
- To assess the biological activities of purified TBSF.
Main Methods:
- Static adsorption experiments to select AB-8 resin for TBSF adsorption.
- Preparative chromatography and liquid chromatography-mass spectrometry (LC-MS) for purification and identification.
- Simulated moving bed (SMB) chromatography for large-scale purification and optimization of parameters.
Main Results:
- AB-8 resin was effective for TBSF adsorption, with optimal parameters identified for preparative chromatography.
- LC-MS analysis identified seven major flavonoids in TBSF, primarily eluted with 40-60% ethanol.
- Optimized SMB chromatography achieved 90% purity and 85% yield of TBSF.
- Purified TBSF exhibited enhanced α-glucosidase inhibition and antioxidant activity compared to raw TBSF and rutin.
Conclusions:
- Simulated moving bed (SMB) chromatography is a viable and efficient method for the industrial-scale purification of TBSF.
- Purified TBSF possess significant biological activities, highlighting their potential for nutraceutical and pharmaceutical applications.
- The SMB technique can be broadly applied to the purification of other valuable compounds from natural products.
