Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Plant Synthetic Biology Takes Off: Bryophytes Onboard with New Tools, Systems, and Opportunities.

ACS synthetic biology·2026

Same author

A simple cell-cycle control system in Marchantia polymorpha provides a framework for understanding plant cell proliferation.

The Plant cell·2026

Same author

Community-building resources for Marchantia-an accessible model plant system.

Plant & cell physiology·2026

Same author

Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.

ACS synthetic biology·2026

Same author

Spatial distribution of isoprenoid enzymes and MpABCG1 transporter influences sesquiterpene accumulation in Marchantia polymorpha oil bodies.

Communications biology·2026

Same author

Transparent PEDOT:PSS/PDMS Leaf Tattoos for Multiplexed Plant Health Monitoring and Energy Harvesting.

Biosensors·2025

Search research articles

Related Experiment Video

Updated: Dec 26, 2025

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Systematic Tools for Reprogramming Plant Gene Expression in a Simple Model, Marchantia polymorpha.

Susanna Sauret-Güeto¹, Eftychios Frangedakis¹, Linda Silvestri^1,2

¹Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.

ACS Synthetic Biology

|March 13, 2020

Summary

This summary is machine-generated.

The OpenPlant toolkit offers new resources and methods to use Marchantia, a simple liverwort, as a plant bioengineering testbed. This toolkit simplifies genetic analysis and synthetic biology applications for researchers without traditional plant biology facilities.

Keywords:

Marchantia OpenPlant chloroplast gene assembly gene editing plant toolkit

More Related Videos

Scalable Transfection of Maize Mesophyll Protoplasts

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Related Experiment Videos

Last Updated: Dec 26, 2025

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

Published on: March 20, 2016

Scalable Transfection of Maize Mesophyll Protoplasts

Scalable Transfection of Maize Mesophyll Protoplasts

Published on: June 23, 2023

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Area of Science:

Plant Biology
Synthetic Biology
Bioengineering

Background:

Marchantia, a liverwort, offers a unique model for plant bioengineering due to its simple morphology, haploid life cycle, and open development.
Existing methods for plant research often require specialized facilities, limiting accessibility.
There is a need for standardized tools and techniques to facilitate plant bioengineering research.

Purpose of the Study:

To present the OpenPlant toolkit, a comprehensive set of resources and techniques for developing Marchantia as a plant bioengineering testbed.
To introduce novel methods for Marchantia propagation and maintenance, reducing the need for glasshouse facilities.
To establish standardized DNA elements and promoter sequences for use in Marchantia bioengineering.

Main Methods:

Development of efficient axenic propagation and maintenance techniques for Marchantia.
Creation of robust Loop assembly vector systems for nuclear and chloroplast transformation and genome editing.
Screening of transcriptomic data to identify and validate novel constitutive promoter elements.

Main Results:

Marchantia can be easily propagated and amplified, facilitating large-scale experiments.
Established standardized DNA elements and vector systems for nuclear and chloroplast engineering.
Identified and validated new constitutive promoter elements through transcriptomic analysis and in vivo testing.
The OpenPlant toolkit provides accessible resources for plant bioengineering.

Conclusions:

The OpenPlant toolkit enables Marchantia to serve as an accessible and effective testbed for plant bioengineering and synthetic biology.
The developed resources and techniques simplify genetic manipulation and analysis in Marchantia.
This initiative promotes the establishment of common standards in plant synthetic biology research.