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Published on: May 17, 2019
Expression analysis of growth arrest specific 8 and its anti-sense in breast cancer tissues
Sepideh Dashti1, Zahra Taherian-Esfahani1, Vahid Kholghi-Oskooei2
1Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:
The Growth arrest specific 8 (GAS8) and its anti-sense transcript (GAS8-AS1) are located in a genomic region that is frequently mutated in breast cancer. These transcripts have established tumor suppressor effects in some human malignancies. In the current investigation, we aimed at identification of the role of GAS8 and GAS8-AS1 in breast cancer. We measured gene expression of GAS8 and GAS8-AS1 in paired tumoral and non-tumoral tissues obtained from 88 breast cancer patients by means of real time PCR. No significant differences were identified in expressions of GAS8 and GAS8-AS1 in tumoral samples compared with non-tumoral tissues (Fold changes = 1.53 and 1.71; P values = .28 and 0.14 respectively). Transcript levels of GAS8-AS1 was significantly correlated with estrogen receptor (ER) status (P = .01). Expression of GAS8 gene was associated with history of oral contraceptive use (P = .04). The similar expressions of GAS8 and GAS8-AS1 genes in tumoral and non-tumoral tissues of breast in spite of previous reports regarding their fundamental tumor suppressor roles in other tissues show that these genes are not involved in the pathogenesis of breast cancer. So, these genes have distinct roles in diverse tissues.
Insights
Growth arrest specific 8 (GAS8) and GAS8-AS1 show no significant expression differences in breast tumors compared to normal tissues. These genes do not appear to be involved in breast cancer development.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Growth arrest specific 8 (GAS8) and its antisense transcript (GAS8-AS1) are located in a frequently mutated region in breast cancer.
- These genes have demonstrated tumor suppressor functions in other human cancers.
Purpose of the Study:
- To investigate the role of GAS8 and GAS8-AS1 in breast cancer pathogenesis.
- To analyze the expression levels of GAS8 and GAS8-AS1 in breast tumor tissues versus adjacent non-tumoral tissues.
Main Methods:
- Gene expression analysis of GAS8 and GAS8-AS1 using real-time PCR.
- Comparison of transcript levels in 88 paired breast tumor and non-tumoral tissue samples.
Main Results:
- No significant differences in GAS8 and GAS8-AS1 expression were observed between tumoral and non-tumoral breast tissues (Fold changes = 1.53 and 1.71; P values = 0.28 and 0.14).
- GAS8-AS1 transcript levels showed a significant correlation with estrogen receptor (ER) status (P = 0.01).
- GAS8 gene expression was associated with a history of oral contraceptive use (P = 0.04).
Conclusions:
- GAS8 and GAS8-AS1 do not appear to play a significant role in the pathogenesis of breast cancer, despite their known tumor suppressor functions in other malignancies.
- The distinct roles of GAS8 and GAS8-AS1 in different tissues suggest tissue-specific functions.
