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Updated: Dec 26, 2025
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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Published on: October 27, 2020
4.8K
Analysing the nanoparticle-protein corona for potential molecular target identification
Chandra Kumar Elechalawar1, Md Nazir Hossen1, Lacey McNally2
1Department of Pathology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Summary
Nanoparticles form a protein corona (PC) in biological systems, offering insights into nanoparticle behavior and disease mechanisms. This review explores using nanoparticles for identifying disease targets through PC analysis.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Proteomics
Background:
- Host proteins associate with nanoparticles, forming a "protein corona" (PC).
- PC composition provides data on nanoparticle interactions, biodistribution, toxicity, and clearance.
- Analyzing PC in patient samples can reveal disease-specific proteomes and therapeutic targets.
Purpose of the Study:
- To review target identification using nanoparticles in biological samples.
- To summarize the formation, characteristics, and analysis of the protein corona.
- To highlight the advantages of nanotechnology for discovering diagnostic and therapeutic targets.
Main Methods:
- Review of physicochemical interactions influencing protein corona formation.
- Analysis of nanoparticle parameters (size, charge, surface modification) on PC.
- Discussion of challenges in PC analysis in biological fluids.
Main Results:
- Nanoparticles serve as tools for identifying disease-related molecular targets.
- Protein corona formation is influenced by nanoparticle properties and environmental factors.
- Nanotechnology offers advantages over traditional methods for target identification.
Conclusions:
- Nanoparticle-protein corona analysis is a promising strategy for disease target discovery.
- Understanding PC formation is crucial for optimizing nanoparticle-based diagnostics and therapeutics.
- Nanotechnology provides a powerful platform for advancing precision medicine.
