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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Published on: October 17, 2018
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Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes
Benjamin J Ravenhill1, Lior Soday1, Jack Houghton1
1Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK.
Scientific Reports
|March 14, 2020
Summary
This study characterizes the surface proteins of classical monocytes, a key immune cell type. Identifying these proteins helps differentiate monocyte subsets and understand their immune functions.
Area of Science:
- Immunology
- Proteomics
- Cell Biology
Background:
- Monocytes are crucial innate immune cells with distinct subsets: classical, intermediate, and non-classical.
- These subsets differ in surface marker expression (CD14, CD16), phagocytosis, and cytokine secretion.
- Understanding monocyte subset phenotypes is vital for insights into immune function and disease states.
Purpose of the Study:
- To characterize the surface proteome of classical monocytes.
- To identify novel protein markers differentiating monocyte subsets.
- To enhance understanding of monocyte subset phenotype and function.
Main Methods:
- Utilized highly multiplexed Tandem-Mass-Tag (TMT)-based mass spectrometry.
- Employed selective cell surface biotinylation for protein enrichment.
- Interrogated phenotypic differences across monocyte subsets.
Main Results:
- Successfully characterized the classical monocyte surface proteome.
- Identified potential novel protein markers for monocyte subset discrimination.
- Provided a detailed proteomic profile of classical monocytes.
Conclusions:
- The study provides a comprehensive surface proteome map of classical monocytes.
- Identified protein markers can aid in distinguishing monocyte subsets.
- This research offers valuable insights into monocyte heterogeneity and function.
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